Production of lactic acid (d-al) from hass seeds by the lactogenic eca escherichia coliju1
DOI:
https://doi.org/10.24054/limentech.v21i1.2364Keywords:
Azúcares reductores, hidrólisis ácida, Escherichia coli JU15, semilla de aguacate, fermentaciónAbstract
The main objective was the study and physical-chemical characterization of Hass avocado seed (residue to be used as a source of carbohydrates) for the production of lactic acid (D-Lactic Acid) together with the lactogenic strain Escherichia coliJU15, optimal conditions of time and concentration of sulfuric acid (H2SO4) were set as acid pretreatment applied to the starch extracted from the seed for the recovery of reducing sugars. Characterization of lignocellulosic materials included quantification of moisture, extractives, structural carbohydrates, starch, lignin and ash, in order to determine the transformation potential of the seed. The quantification and identification of reducing sugars was carried out by HPLC. Subsequently, fermentation was carried out with the E. coliJU15 strain on the starch hydrolysate of the seeds with AM1 mineral medium. The main compound that was identified was glucose, on the other hand, a minimum amount of acetic acid, xylose and arabinose which formed the hydrolyzate, finally a production of 70.28 gD-AL/L was obtained in 72 hours of fermentation with a volumetric productivity of 0.97 gD-AL/L*h and a maximum yield of 96.37% of the final product.
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