Comparison of two green technologies for the control of methicillin-resistant Staphylococcus aureus growth.

Authors

  • Raquel A. Villamizar Gallardo Universidad de Pamplona
  • Karla Cruz Cuellar Universidad de Pamplona
  • Edgar Emir González Jiménez Pontificia Universidad Javeriana
  • Johann F. Osma Universidad de los Andes

DOI:

https://doi.org/10.24054/limentech.v20i1.1467

Keywords:

Antibacterial agents, nanoparticles, silver, volatile oils

Abstract

Methicillin-resistant Staphylococcus aureus(MRSA) is a pathogenic bacterium that according to the World Health Organization (WHO) should be included in the list of priority pathogens towards which new treatment mechanisms should be developed, given its resistance to antibiotics. Objective:In thisstudy, the bactericidal effect of two products obtained by green chemistry such as essential oils and silver nanoparticles, were compared against a MRSA strain isolated from food. Methods.The evaluation of the inhibition of the bacteria was based on diskdiffusion tests and well plate microdilution in base culture media modified with three different types of essential oils and two types of silver nanoparticles as bactericidal agents. In addition, scanning electron microscopy (SEM) was used to visualize damage at the structural level of the bacteria. Results:The results allowed determining that the essential oil Lippia origanoidesexhibited greater efficacy in inhibiting the growth of MRSA, with a minimum inhibitory concentration and a minimum bactericidalconcentration of 2 mg / ml. On the contrary, silver nanoparticles showed little significant effect in reducing and / or inhibiting the growth of the studied pathogen. Microscopically, it was possible to observe total loss of the bacteria cell wall integrity. Conclusions.It was possible to determine that essential oils were more efficient than silver nanoparticles inhibiting MRSA and, therefore, could be used as a green effective alternative to control pathogenic bacteria growth in food matrices.

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Published

2022-09-23 — Updated on 2022-06-30

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How to Cite

Villamizar Gallardo, R. A., Cruz Cuellar, K., González Jiménez, E. E., & Osma, J. F. (2022). Comparison of two green technologies for the control of methicillin-resistant Staphylococcus aureus growth. @limentech, Ciencia Y Tecnología Alimentaria, 20(1), 5–20. https://doi.org/10.24054/limentech.v20i1.1467 (Original work published September 23, 2022)

Issue

Vol. 20 No. 1 (2022)

Section

Artículos