Determination of polyphenoloxidase activity and inhibition in lulo (Solanum quitoense lam)
DOI:
https://doi.org/10.24054/limentech.v8i2.1521Keywords:
absorbance, enzyme, inhibition, lulo, polyphenoloxidaseAbstract
The objective of the research was to determine the influence of the use of phenolic precipitants and inhibitors on the activity of the enzyme polyphenoloxide (PFO), as well as to determine which part of the fruit, skin or pulp, has a greater activity of this enzyme. Work was done on crude extracts, both pulp and skin of the lulo, in whose extraction the use of phenolic precipitants was included
TRITON and polyvinylpyrrylidone (PVPP), in order to establish whether these allow obtaining an extract with greater enzymatic activity. Likewise, the use of antioxidants, citric acid and sodium metabisulfite in two concentrations (500 and 1000 ppm) was evaluated. As a result, it was obtained that the best condition for the extraction of the polyphenoloxidase enzyme is the use of 3% newt, obtaining a greater presence of this enzyme in the pil; in which the sodium metabisulfite (1000 ppm) manages to inhibit its activity by 56.08%.
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