Effect of three soybean flavonoids on the midgut and larval survival of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

Authors

DOI:

https://doi.org/10.24054/cyta.v6i2.2306

Keywords:

Histopathology, midgut epithelium, insect intestinal epithelium, synergism

Abstract

Morphological changes in Anticarsia gemmatalis Hübner caterpillar midgut, development and mortality feeding on soybean and artificial diets containing flavonoids were evaluated. Soybean flavonoid concentrations were measured, and artificial diets prepared to add synthetic flavonoids at same soybean proportions. Anticarsia gemmatalis caterpillars fed on susceptible and resistant soybean and artificial diets were submitted to histological analysis. Survival, development period and weight were accounted. Flavonoids daidzein, quercetin, and rutin were found in susceptible (IAC-PL1) and resistant (IAC-17, IAC-24) soybean. Only daidzein expressed high concentrations on resistant varieties. Anticarsia gemmatalis survival was lower feeding on resistant soybean and an artificial diet containing the three flavonoids. Caterpillars got less mass growth feeding on resistant than susceptible soybean. Artificial diets added with flavonoids did not have these effects and any treatment affected Anticarsia gemmatalis developing periods. Midgut cells were damaged, but not the peritrophic membrane and the muscular wall of the caterpillar midgut. The IAC-PL1 soybean deformed columnar cells and reduced the number of regenerative ones. The IAC-17 caused columnar cells rupturing and goblet cells deformation. The IAC-24 epithelium detachment from the muscular wall and increased vacuoles in the cytoplasm of the columnar cell. Artificial diets added with flavonoids caused similar changes in midgut morphology as those caused by resistant soybeans. Resistant soybean varieties damaged Anticarsia gemmatalis caterpillar's midgut, causing high mortality and decreased growth mass. Different daidzein, quercetin, and rutin concentrations, in these cultivars, may function synergistically against Anticarsia gemmatalis caterpillars as shown by the effects of artificial diet added with these three flavonoids

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Published

2021-09-25 — Updated on 2021-09-25

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How to Cite

Rios-Diez, J. D., Solis Vargas, M. A., da-Silva, P., Gomez Arrieta, J. D., Martínez , J. A., Serrão, J. E., & Almedia Oliveira, M. G. (2021). Effect of three soybean flavonoids on the midgut and larval survival of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae). CIENCIA Y TECNOLOGÍA AGROPECUARIA, 6(2), 45–57. https://doi.org/10.24054/cyta.v6i2.2306

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Artículos de investigación originales (Scientific Articles)