Effect of three soybean flavonoids on the midgut and larval survival of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)
DOI:
https://doi.org/10.24054/cyta.v6i2.2306Keywords:
Histopathology, midgut epithelium, insect intestinal epithelium, synergismAbstract
Morphological changes in Anticarsia gemmatalis Hübner caterpillar midgut, development and mortality feeding on soybean and artificial diets containing flavonoids were evaluated. Soybean flavonoid concentrations were measured, and artificial diets prepared to add synthetic flavonoids at same soybean proportions. Anticarsia gemmatalis caterpillars fed on susceptible and resistant soybean and artificial diets were submitted to histological analysis. Survival, development period and weight were accounted. Flavonoids daidzein, quercetin, and rutin were found in susceptible (IAC-PL1) and resistant (IAC-17, IAC-24) soybean. Only daidzein expressed high concentrations on resistant varieties. Anticarsia gemmatalis survival was lower feeding on resistant soybean and an artificial diet containing the three flavonoids. Caterpillars got less mass growth feeding on resistant than susceptible soybean. Artificial diets added with flavonoids did not have these effects and any treatment affected Anticarsia gemmatalis developing periods. Midgut cells were damaged, but not the peritrophic membrane and the muscular wall of the caterpillar midgut. The IAC-PL1 soybean deformed columnar cells and reduced the number of regenerative ones. The IAC-17 caused columnar cells rupturing and goblet cells deformation. The IAC-24 epithelium detachment from the muscular wall and increased vacuoles in the cytoplasm of the columnar cell. Artificial diets added with flavonoids caused similar changes in midgut morphology as those caused by resistant soybeans. Resistant soybean varieties damaged Anticarsia gemmatalis caterpillar's midgut, causing high mortality and decreased growth mass. Different daidzein, quercetin, and rutin concentrations, in these cultivars, may function synergistically against Anticarsia gemmatalis caterpillars as shown by the effects of artificial diet added with these three flavonoids
Downloads
References
Almeida GD De, Zanuncio JC, Pratissoli D, Polanczyk RA, Azevedo DO, and Serrão JE, (2014). Cytotoxicity in the midgut and fat body of Anticarsiagemmatalis(Lepidoptera : Geometridae ) larvae exerted by neem seeds extract, Invertebrate Survival Journal 11:79–86
Anshul N, Bhakuni RS, Gaur R, and Singh D, (2013). Isomeric flavonoids of Artemisia annua (Asterales: Asteraceae) as insect growth inhibitors against Helicoverpa armigera (Lepidoptera: Noctuidae), Florida Entomology 96:897–903
Barbehenn R V. and KochmanskiJ, (2013). Searching for synergism: effects of combinations of phenolic compounds and other toxins on oxidative stress in Lymantria dispar caterpillars, Chemoecology 23:219–231
Bernardi O, Malvestiti GS, Dourado PM, Oliveira WS, Martinelli S, Berger GU, et al., (2012). Assessment of the high-dose concept and level of control provided by MON 87701 × MON 89788 soybean against Anticarsia gemmatalisand Pseudoplusia includens (Lepidoptera: Noctuidae) in Brazil, Pest Management Science 68:1083–1091
Binder BF and Bowers WS, (1994). Precocene II-induced changes in the anatomy of midgut goblet cells of last-instar larvae of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), International Journal of Insect Morphology and Embryology 23:127–134
Brito AF de, Braconi CT, Weidmann M, Dilcher M, Alves JMP, Gruber A, et al., (2015). The pangenome of the Anticarsia gemmatalismultiple nucleopolyhedrovirus (AgMNPV), Genome Biology and Evolution 8:94–108
Broussalis AM, Clemente S, and Ferraro GE, (2010). Hybanthusparviflorus (Violaceae): Insecticidal activity of a South American plant, Crop Protection 29:953–956
Céspedes CL, Salazar JR, Ariza-Castolo A, Yamaguchi L, Ávila JG, Aqueveque P, et al., (2014). Biopesticides from plants: Calceolaria integrifolia s.l., Environment Research 132:391–406
Chiang AS, Yen DF, and Peng WK, (1986). Defense reaction of midgut epithelial cells in the rice moth larva (Corcyra cephalonica) infected with Bacillus thuringiensis, Journal of Invertebrate Pathology 47:333–339
Conab -Companhia Nacional de Abastecimento-Observatório Agrícola. (2021, 7 October). Acompanhamento da Safra Agrícola; Grãos. V.5 Safra 2021/2022. https://www.conab.gov.br/info-agro/safras/graos. [Accessed 15 Oct. 2021]
de Freitas Bueno RCO, de Freitas Bueno A, Moscardi F, Postali Parra JR, and Hoffmann-Campo CB, (2011). Lepidopteran larva consumption of soybean foliage: basis for developing multiple-species economic thresholds for pest management decisions, Pest Management Science 67:170–174
Eisemann CH and Binnington KC, The peritrophic membrane: Its formation, structure, chemical composition and permeability in relation to vaccination against ectoparasitic arthropods, International Journal of Parasitology 24:15–26 (1994).
Fiat Muhammad, Martínez Luis Carlos, Plata-Rueda Angelica, Gonzaga Gonçalves Wagner, Shareef Muhammad, Zanuncio Jose Cola, Serrão Jose Eduardo. Toxicological and morphological effects of tebufenozide on Anticarsia gemmatalis(Lepidoptera: Noctuidae) larvae. Chemosphere. 212: 337-345 https://doi.org/10.1016/j.chemosphere.2018.08.088 (2018).
Gazzoni DL, Hülsmeyer A, and Hoffmann-Campo CB, (1997). Effect of diferente rates of quercetin and rutin on the biology of Anticarsia gemmatalis, Pesquisa Agropecuária Brasileira 32:673–681
Gomes FM, Carvalho DB, Machado EA, and Miranda K, (2013). Ultrastructural and functional analysis of secretory goblet cells in the midgut of the lepidopteran Anticarsia gemmatalis, Cell Tissue Research 352:313–326
Grella Tatiane Caroline, Soares-Lima Hellen Maria, Malaspina Osmar, Ferreira Nocelli Roberta Cornélio (2019). Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach. Chemosphere 236:124255
Hay William T., Behle Robert W., Berhow MarkA., Miller Andie C., Selling GordonW. (2020). Biopesticide synergy when combining plant flavonoids and entomopathogenic baculovirus. Scientific Reports 10:6806 https://doi.org/10.1038/s41598-020-63746-6
Jadhav DR, Mallikarjuna N, Rathore A, and Pokle D. (2012). Effect of some flavonoids on survival and development of Helicoverpa armigera (Hubner) and Spodoptera litura (Fab) (Lepidoptera: Noctuidae), Asian Journal of Agricultural Science 4:298–307
John KMM, Jung ES, Lee S, Kim J-S, and Lee CH. (2013). Primary and secondary metabolites variation of soybean contaminated with Aspergillus sojae, Food Research International 54:487–494
Karowe DN and Radi JK. (2011). Are the phytoestrogens genistein and daidzein anti-herbivore defenses? A test using the gypsy moth (Lymantria dispar), Journal Chemestry Ecology 37:830–837
Kim JK, Kim E-H, Park I, Yu B-R, Lim JD, Lee Y-S, et al. (2014). Isoflavones profiling of soybean [Glycine max(L.) Merrill] germplasms and their correlations with metabolic pathways, Food Chemestry 153:258–264
Knaak N and Fiuza LM. (2005). Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki, Brazilian Journal Microbiology 36:196–200
Levy SM, Falleiros AMF, Moscardi F, and Gregório EA. (2011). The role of peritrophic membrane in the resistance of Anticarsia gemmatalis larvae (Lepidoptera: Noctuidae) during the infection by its nucleopolyhedrovirus (AgMNPV), Arthropod Struct Development 40:429–434
Levy SM, Falleiros AMF, Moscardi F, Gregório EA, and Toledo LA. (2008). Ultramorphology of digestivie tract of Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae) at final larval development, Semina Ciencias Agrarias 29:313–322
Levy SM, Moscardi F, Falleiros AMF, Silva RJ, and Gregório EA. (2009). A morphometric study of the midgut in resistant and non-resistant Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae to its nucleopolyhedrovirus (AgMNPV), Journal of Invertebrate Pathology 101:17–22
Lourencao AL, Reco PC, Braga NR, Valle JGE, Pinheiro JB. (2010). Yield of soybean genotypes under infestation of the velvetbean caterpillar and stink bugs, Neotropical Entomology 39: 275–281
Magarelli G, Lima LHC, da Silva JG, SouzaDe JR, and de Castro CSP. (2014). Rutin and totalisoflavone determination in soybean at different growth stages by using voltammetric methods, Microchemical Journal 117:149–155
Nascimento IR, Murata AT, Bortoli SA, and Lopes LMX. (2003). Insecticidal activity of chemical constituents from Aristolochia pubescens against Anticarsia gemmatalislarvae, Pest Management Science 60:413–416
Navickiene HMD, Miranda JE, Bortoli SA, Kato MJ, Bolzani VS, and Furlan M. (2007). Toxicity of extracts and isobutyl amides from Piper tuberculatum: potent compounds with potential for the control of the velvetbean caterpillar, Anticarsia gemmatalis, Pest Management Science 63:399–403
Nenaah GE. (2013). Potential of using flavonoids, latex and extracts from Calotropis procera (Ait.) as grain protectants against two coleopteran pests of stored rice, Industrial Crops and Products 45:327–334
Downloads
Published
Versions
- 2021-09-25 (3)
- 2021-09-25 (2)
- 2021-09-25 (1)
How to Cite
Issue
Section
License
Copyright (c) 2021 Juan D Rios Díez, Manuel A Solís Vargas, Paulo L da Silva, Jenny D Gomez Arrieta, Juliana A Martínez , Jose E Serrão, Maria G Almedia Oliveira
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
