Caracterización de Pasteurella multocida subsp multocida en la neumonía porcina
DOI:
https://doi.org/10.24054/limentech.v13i1.1596Palabras clave:
Cerdos, Neumonía, Pasteurella, multocida subsp multocida, PCRResumen
A partir de 181 muestras obtenidas de pulmones de cerdos con lesiones de neumonía recolectadas en el matadero de León, durante los años 2003-2004 y de otras 76 cepas aisladas previamente, durante 1987-1988; procedentes de diversas provincias de Castilla y León, se logró caracterizar fenotípica y genotípicamente como Pasteurella multocida, para un total de 221 cepas bacterianas. Mediante la identificación bioquímica estas cepas se sub-tipificaron como subespecie multocida, siendo agrupadas en los tipos capsulares A y D, mediante el test de sensibilidad a la hialuronidasa y de aglutinación de la acriflavina, respectivamente. En la caracterización genotípica a nivel de especie se amplificó mediante PCR la secuencia génica KMT1, e igualmente la diferenciación de los tipos capsulares (A, B, D, E y F) se realizó mediante el análisis de la región 2 del cromosoma, siendo estos resultados, correspondientes con la caracterización capsular A y D.
Citas
Ackermann, M., DeBey M., Register K., Larson, D., Kinyon J. Tonsil and turbinate colonization by toxigenic and nontoxigenic strains of Pasteurella multocida in conventionally raised swine. (1994). Journal of Veterinary Diagnostic Investigation. 6: 375–
Avril, J. L., Donnio, P. Y., Pouedras, P. Selective Medium for Pasteurella multocida and Its Use to Detect Oropharyngeal Carriage in Pig Breeders. (1990). Journal of Clinical Microbiology. 28(6): 1438-40.
Vera L. Yanedt A. et al. /@limentech, Ciencia y Tecnología Alimentaria 13(2015) 5-12
Bisgaard, M., Houston, S. B., Mutters, R., Stenzel, A. Reclassification of German, British and Dutch isolates of so- called Pasteurella multocida obtained from pneumonic calf lungs. (1991). Veterinary Microbiology. 26, 115-124.
Blackall, P. J., Fegan, N., Chew, G. T. I., Hampson, D. J. Population structure and diversity of avian isolates of Pasteurella multocida from Australia. (1998). Microbiology. 144: 279–289.
Blackall, P.J., Pahoff, J.L., Bowles, R. Phenotypic characterisation of Pasteurella multocida isolates from Australian pigs. (1997). Veterinary Microbiology. 57: 355-360.
Blackall, P.J., Pahoff, J.L., Marks, D., Fegan, N., Morrow, C.J. Characterisation of Pasteurella multocida isolated from fowl cholera outbreaks on turkey farms. (1995). Australian Veterinary Journal. 72: 135-138.
Carter, G. R., Rundell, D. W. Identification of type A strains of Pasteurella multocida using Staphylococcal hyaluronidase. (1975). Veterinary Record. 87: 343.
Carter, G.R., Subpronto, P. Identification of type D strains of Pasteurella multocida with acriflavine. (1973). American Journal of Veterinary Research. 34: 293-294.
Cowan, S.T., Steel, K.J. Manual for the Identification of Medical Bacteria. Third edition Cambridge Univ. Press, UK: In: Barrow, G. I., Feltham, R. K. A. (Eds.).(1993). p117–148.
Djordjevic, S. P., Eamens, G. J., Ha, H., Walker, M. J., Chin, J.
C. Demonstration that Australian Pasteurella multocida isolates from sporadic outbreaks of porcine pneumonia are non- toxigenic (toxA-) and display heterogeneous DNA restriction endonuclease profiles compared with toxigenic isolates from herds with progressive atrophic rhinitis. (1998). Journal Medical Microbiology. 47: 679-688.
Dziva, F., Christensen, H., van Leengoed, L. A., Mohan, K., Olsen, J. E. Differentiation of Pasteurella multocida isolates from cases of atrophic rhinitis in pigs from Zimbabwe by RAPD and ribotyping. (2004).Veterinary Microbiology. 102: 117-122.
Fegan, N., Blackall, P. J., Pahoff, J. L. Phenotypic characterization of Pasteurella multocida isolates from Australian poultry. (1995). Veterinary Microbiology. 47: 281–
Garrity, G. M. Bergey´s Manual of Systematic Bacteriology. Second edition. Volumen two. Michigan State University: Editorial Springer. 2005, p850-865.
Glorioso, J. C., Jones, G. W., Rush, H. G., Pentler, L. J., Darif C. A., Coward, J. E. Adhesion of type A Pasteurella multocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections. (1982). Infection and Immunity. 35: 1103–1109.
Gutiérrez, C. B., Vera, Y. A., Del rio, G. M., Rodríguez, F. E. Procesos producidos por Pasteurella multocida. (2005). Suis. 14: 30-41.
Iwamatsu, S., Sawada, T. Relationship between serotypes, dermonecrotic toxin production of Pasteurella multocida isolates and pneumonic lesions of porcine lung. (1988). Nippon Juigaku. Zasshi 50: 1200-1206.
Jamaludin, R., Blackall, P. J., Hansen, M. F., Humphrey, S., Styles, M. Phenotypic and genotypic characterisation of Pasteurella multocida isolated from pigs at slaughter in New Zealand. (2005). New Zealand Veterinary Journal. 53(3): 203-
Kasten, R. W., Wakenell, P. S., Ahmad, S., Yilma, T. D., Hirsh,
D. C. Lack of protection against avian cholera by vaccination with recombinant P6-like protein from Pasteurella multocida. (1997). Avian Diseases. 41: 972-976.
Korbel. R., Gerlach, H., Bisgaard, M., Hafez, H. M. Further investigations on Pusteurellu multocidu infections in feral birds injured by cats. (1992). Journal of Veterinary Medicine. 39: 10- 18.
Lariviere, S., Leblanc, L., Mittal, K. R., y Martineau, G. P. Characterization of Pasteurella multocida from nasal cavities of piglets from farms with or without atrophic rhinitis. (1992). Journal Clinical Microbiology. 30: 1398-1401.
Letellier, A., Dubreuil, D., Roy, G., Fairbrother, J. M., Jacques. M. Determination of affinity of Pasteurella multocida isolates for porcine respiratory tract mucus, and partial characterization of the receptors. (1991). American Journal of Veterinary Research. 52:34-39.
López, A. G., Polanco, G., De Mora, Y. P., De Aponte, F. M. Tipos capsulares A y D de Pasteurella multocida de origen
porcino. (1988). Revista de la Facultad de Ciencias Veterinarias U.C.V. 35 (1-4): 21-28.
Mohan, K., Sadza, M., Madsen, M., Hill, F.W.G., Pawandiwa, A. Phenotypic characterization of Zimbabwean isolates of Pasteurella multocida. (1994). Veterinary Microbiology. 38:
-357.
Mutters, R., Ihm, P., Pohl, S., Fredericksen, W., Mannheim, W. Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. (1985). International Journal of Systematic Bacteriology. 35: 309-322.
Nielsen, J. P., Frederiksen, W. Atrophic rhinitis in pigs caused by a human isolate of toxigénica Pasteurella multocida. 1990. In: International pig veterinary society congress, 11. Proceedings, 1989, p75.
Petersen, K. D., Christensen, H., Bisgaard, M., Olsen, J. E. Genetic diversity of Pasteurella multocida isolated from fowl cholera as demonstrated by ribotyping, 16S rRNA and partial atpD sequence comparisons. (2001). Microbiology. 147: 2739–
Pijoan, C.. Pneumonic Pasteurellosis. 7 ed. Iowa; Wolfe Publshing Ltd: In Leman, A. (Eds) Diseases of Swine. 1992, p552-559.
Pijoan, C., Morrison, R. B., Hilley, H. D. Serotyping of Pasteurella multocida Isolated from Swine Lungs Collected at Slaughter. (1983). Journal of Clinical Microbiology. 17(6): 1074-1076.
Prescott, J.F, Bhasin, J.L., Sanford, S. E. Serotypes and antimicrobial susceptibility of Pasteurella multocida isolated from cattle and pigs in Ontario. (1984). The Canadian Veterinary Journal. 25: 117-118.
Rhoades, K. R., Rimler, R. B. Fowl Cholera. In: Pasteurella and Pasteurellosis. London: Academic Press Limited, 1989, p95– 144.
Rutter, J. M. Virulence of Pasteurella multocida in athrophic rhinitis of gnotobiotic pigs infected with Bordetella bronchiseptica. (1983). Research in Veterinary Science. 34: 285-287.
Sambrook J., Frisch EF, Maniatis T. Molecular cloning a laboratory manual. 2 edition. New York: Greene Publishing Associates and John Wiley & Sons. 1989.
Shewen, P. E., Rice Conlon, J. A Pathogenesis of bacterial infections in animals, 2nd. Pasteurella. In C. L. Gyles and C. O. Thoen (ed.), ed. Iowa State University Press, Ames. 1993, p216-225.
Shivachandra, S. B., Kumar, A. A., Chaudhuri, Molecular characterization of Avian strains of Pasteurella multocida serogroup-A: 1 based on amplification of repetitive regions by PCR. (2008).Comparative Immunology, Microbiology & Infectious Diseases. 31(1): 47-62.
Sneath, P. H. A., Stevens, M. Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. nov., Pasteurella mairi sp. nov., and Pasteurella trehalosi sp. Nov. (1990). International Journal of Systematic Bacteriology. 40: 148–153.
Townsend, K. M., Boyce, J. D., Chung, J. Y., Frost, A. J., Adler,
B. Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. (2001). Journal of Clinical Microbiology. 39: 924–929.
Townsend, K. M., Frost, A. J., Lee, C. W.: Papadimitriou, J. M., Dawkins, H. J. Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates. (1998). Journal of Clinical Microbiology. 36: 1096-1100.
Weaver N, Collins M. Evaluation of seven commercial oxidase test products with Pasteurella. (1982). American Journal of Veterinary Research. 43 (2):363-4.
