In vitro antagonistic activity of native Trichoderma strains against Botrytis sp
DOI:
https://doi.org/10.24054/bistua.v23i1.3660Keywords:
Biological control, Phytopathogen, Agrochemicals, Grey mouldAbstract
Botrytis cinerea is a plant pathogen that affects a wide range of plants and is responsible for severe economic losses in more than 200 crops worldwide. Although the use of fungicides is the most common strategy for its control, the genetic plasticity of this fungus allows it to develop resistance. Consequently, biological control has gained ground as an alternative or complementary approach to fungicides. In this respect, the genus Trichoderma is the most extensively studied group with the greatest potential for biological control of B. cinerea. Therefore, the search and study of native Trichoderma strains with biocontrol potential is a line of interest for the local and regional agricultural sector. This study reports the in vitro investigation of the biocontrol capacity of a commercial product, in comparison with the antagonistic power of two native strains isolated from soils in the agricultural areas of the municipalities of Pamplona (TN01) and Bucarasica (TN02), in Norte de Santander. Morphological characterisation determined that both strains are taxonomically located in the Viride clade of the genus Trichoderma. The growth rate and the percentage of radial growth inhibition (PICR) of the antagonists were determined using the dual culture technique. The results indicated that TN01 was the strain with the highest potential to be used as a biological controller of Botrytis spp. This strain showed the highest apical growth rate and achieved a PICR of 80% at the end of the experiment, exceeding that achieved by the commercial reference product. Conversely, strain TN02 exhibited a diminished antagonistic capacity, with a PICR of 55% after a 12-day period. This observation suggests the potential for the autochthonous strain TN01 to be evaluated under cultivation conditions.
Downloads
References
Aldana Bohórquez, S.M. and García Rico, R.O. 2019. Efecto de diferentes condiciones de estrés sobre el crecimiento vegetativo del hongo filamentoso Acremonium chrysogenum. BISTUA REVISTA DE LA FACULTAD DE CIENCIAS BASICAS. 17, 2 (Aug. 2019), 182–195. https://doi.org/10.24054/bistua.v17i2.248.
Azevedo, D.M.Q., Martins, S.D.S., Guterres, D.C., Martins, M.D., Araújo, L., Guimaraes, L.M.S., Alfenas, A.C., Furtado, G.Q., 2020. Diversity, prevalence and phylogenetic positioning of Botrytisspecies in Brazil. Fungal Biol. 124, 940–957. https://doi.org/10.1016/j.funbio.2020.08.002
Brankica, T., Delibasic, G., Milivojevic, J., Nikolic, M., 2009. Characterization of Botrytis cinereaisolates from small fruits and grapevine in Serbia. Arch. Biol. Sci. 61. https://doi.org/10.2298/ABS0903419T
Cheung, N., Tian, L., Liu, X., Li, X., 2020. The Destructive Fungal Pathogen Botrytis cinerea Insights from Genes Studied with Mutant Analysis. Pathog. Basel Switz. 9, E923. https://doi.org/10.3390/pathogens9110923
Cruz, A.F., Barka, G.D., Sylla, J., Reineke, A., 2018. Biocontrol of strawberry fruit infected by Botrytis cinerea: Effects on the microbial communities on fruit assessed by next generation sequencing. J. Phytopathol. 166, 403–411. https://doi.org/10.1111/jph.12700
Debnath, S., Chakraborty, G., Dutta, S.S., Chaudhury, S.R., Das, P., Saha, A.K., 2020. Potential of Trichodermaspecies as biofertilizer and biological control on Oryza sativa L. cultivation. Biotecnol. Veg. 20, 1–16.
Díaz de la Osa A, Almenares Casanova M, Fernández Millares B, Aguado Casas ME, Rojas L, Zeilinger S, Hernández Rodríguez A. Secondary metabolites and extracellular proteases contribute to the antagonistic action of indigenous Trichoderma strainsagainst Botrytis cinerea. Fungal Biol. 2025 Feb;129(1):101530. doi: 10.1016/j.funbio.2024.101530. Epub 2024 Dec 31. PMID: 39826978.
Elías, R., Arcos, O., Arbeláez, G., 1993. Estudio del antagonismo de algunas especies de Trichodermaaisladas de suelos colombianos en el control de Fusarium oxysporumy Rhizoctonia solani. Agron. Colomb. 10, 52–61.
Fernandes, V.C., Maia, M.L., Correia Sá, L., Sousa, S., Paíga, P., Vera, J.L., Domingues, V.F., Delerue Matos, C., 2021. Extraction Procedures and Chromatography of Pesticides Residues in Strawberries, in: Inamuddin, Ahamed, M.I., Lichtfouse, E. (Eds.), Sustainable Agriculture Reviews 47: Pesticide Occurrence, Analysis and Remediation Vol. 1 Biological Systems, Sustainable Agriculture Reviews. Springer International Publishing, Cham, pp. 167–201. https://doi.org/10.1007/978-3-030-54712-7_5
Fernández, O, D., Grabke, A., Li, X., Schnabel, G., 2015. Independent Emergence of Resistance to Seven Chemical Classes of Fungicides in Botrytis cinerea. Phytopathology 105, 424–432. https://doi.org/10.1094/PHYTO-06-14-0161-R
Fernández, O, D., Li, X.P., Wang, F., Schnabel, G., 2012. First Report of Gray Mold of Strawberry Caused by Botrytis caroliniana in North Carolina. Plant Dis. 96, 914–914. https://doi.org/10.1094/PDIS-12-11-1018-PDN
García Rico, R.O. and Fierro, F., 2017. Papel de las subunidades alfa de proteínas G en los procesos morfogénicos de hongos filamentosos de la división Ascomycota. Rev. Iberoam. Micol. 34, 1–9. https://doi.org/10.1016/j.riam.2016.06.005
Harman, G.E., Kubicek, C.P., 2002. Trichoderma And Gliocladium. Volume 1: Basic Biology, Taxonomy and Genetics. CRC Press.
He, L., Cui, K., Li, T., Song, Y., Liu, N., Mu, W., Liu, F., 2020. Evolution of the Resistance of Botrytis cinereato Carbendazim and the Current Efficacy of Carbendazim Against Gray Mold After Long Term Discontinuation. Plant Dis. 104, 1647–1653. https://doi.org/10.1094/PDIS-11-19-2457-RE
Isaza, L., Zuluaga, Y.P., Marulanda, M.L., 2019. Morphological, pathogenic and genetic diversity of Botrytis cinereaPers. in blackberry cultivations in Colombia. Rev. Bras. Frutic. 41. https://doi.org/10.1590/0100-29452019490
Jaklitsch, W.M., 2009. European species of HypocreaPart I. The green spored species. Stud. Mycol., European species of Part I. The green spored species 63, 1–91. https://doi.org/10.3114/sim.2009.63.01
Jaklitsch, W.M., Voglmayr, H., 2015. Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Stud. Mycol., Hypocrealean lineages of industrial and phytopathological importance 80, 1–87. https://doi.org/10.1016/j.simyco.2014.11.001
Kumari,R., Kumar, V., Koul, B., Abul Farah M, Mishra AK. 2025. Synergistic effects of Trichoderma and biochar on the biocontrol of two soil borne phytopathogens in chickpeas. Front Microbiol. 1;16. doi: 10.3389/fmicb.2025.1583114.
Martinez Y, Ribera J, Schwarze FWMR, De France K. Biotechnological development of Trichoderma based formulations for biological control. Appl Microbiol Biotechnol. 2023 Sep;107(18):5595-5612. doi: 10.1007/s00253-023-12687-x. Epub 2023 Jul 21. PMID: 37477696; PMCID: PMC10439859.
Meng, L., Mestdagh, H., Ameye, M., Audenaert, K., Hofte, M., Van Labeke, M. C., 2020. Phenotypic Variation of Botrytis cinereaIsolates Is Influenced by Spectral Light Quality. Front. Plant Sci. 0. https://doi.org/10.3389/fpls.2020.01233
Mirzaei, S., Goltapeh, E.M., Shams Bakhsh, M., Safaie, N., 2008. Identification of Botrytisspp. on Plants Grown in Iran. J. Phytopathol. 156, 21–28. https://doi.org/10.1111/j.1439-0434.2007.01317.x
Moreno P, D. M. ., Rivera, M. E. ., Delgado R, J. R. ., & Vargas C, M. J. . (2017). Modelacióndel transporte de plaguicidas e insecticidas en suelos de cultivo de arveja con el software Hydrus 1D en vereda el Escorial, Pamplona Norte de Santander. BISTUA REVISTA DE LA FACULTAD DE CIENCIAS BASICAS,15(2),29–35. https://doi.org/10.24054/bistua.v15i2.621
Nessma, E., Mohamed, E., Nadia, A y Yehia, M. 2025. Biocontrol potential of endophytic Trichoderma harzianumAUMC 14897 against Fusarium seedling blight disease in oat. Zawawy et al. BMC Plant Biology. 25:586, 1 15.https://doi.org/10.1186/s12870-025-06517-7
Pei, Y., Tao, Q., Zheng, X., Li, Y., Sun, X., Li, Z., Qi, X., Xu, J., Zhang, M., Chen, H., Chang, X., Tang, H., Sui, L., Gong, G., 2019. Phenotypic and Genetic Characterization of Botrytis cinereaPopulation from Kiwifruit in Sichuan Province, China. Plant Dis. 103, 748–758. https://doi.org/10.1094/PDIS-04-18-0707-RE
Petrasch, S., Knapp, S.J., Kan, J.A.L. van, Blanco Ulate, B., 2019. Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea. Mol. Plant Pathol. 20, 877–892. https://doi.org/10.1111/mpp.12794
Qin, W. T., Zhuang, W. Y., 2016. Seven wood inhabiting new species of the genus Trichoderma(Fungi, Ascomycota) in Viride clade. Sci. Rep. 6, 27074. https://doi.org/10.1038/srep27074
Rabasco, L., Porras, E., Possas, A., Morcillo, R y Pérez, F.2025. Un enfoque predictivo para reducir el desperdicio poscosecha de fresa: Impacto de las condicionesde almacenamiento y la actividad del agua en la germinación de Botrytis cinerea. Investigación alimentaria internacional.210. 1 11 https://doi.org/10.1016/j.foodres.2025.116401
Rodríguez V., R., & Rodríguez B., M. (2019). Biorremediación de suelo de la chinampa ubicada en Cuemanco delegación Xochimilco, D.F contaminado con plaguicidas empleando Penicillium sp. BISTUA REVISTA DE LA FACULTAD DE CIENCIAS BASICAS, 17(1), 48–58. https://doi.org/10.24054/bistua.v17i1.270
Ribeiro, U y Lima, J. 2025. Lecciones de campo: Trichoderma en la agriculturay la salud humana. Revista Canadiense de Microbiología. 71. 1 15. https://doi.org/10.1139/cjm-2024-0227amaniego-Fernández, L.M., Harouna, M., Corbea, O., Rondón Castillo, A.J., Placeres Espinosa, I., Samaniego Fernández, L.M., Harouna, M., Corbea, O., Rondón Castillo, A.J., Placeres Espinosa, I., 2018. Aislamiento, identificación y evaluación de cepas autóctonas de Trichodermaspp. antagonistas de patógenos del suelo. Rev. Protección Veg. 33.
Samuels, G.J., Dodd, S.L., Lu, B. S., Petrini, O., Schroers, H. J., Druzhinina, I.S., 2006. The Trichoderma koningiiaggregate species. Stud. Mycol. 56, 67–133. https://doi.org/10.3114/sim.2006.56.03
Torrent C, Gil Durán C, Rojas Aedo JF, Medina E, Vaca I, Castro P, García Rico RO, Cotoras M, Mendoza L, Levicán G, Chávez R. Role of sfk1 Gene in the Filamentous Fungus Penicillium roqueforti. Front Microbiol. 2017 Dec 6;8:2424. doi: 10.3389/fmicb.2017.02424. PMID: 29270163; PMCID: PMC5723657.
Woo SL, Hermosa R, Lorito M, Monte E. Trichoderma: a multipurpose, plant beneficial microorganism for eco sustainable agriculture. Nat Rev Microbiol. 2023 May;21(5):312 326. doi: 10.1038/s41579-022-00819-5. Epub 2022 Nov 22. PMID: 36414835.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Cristian Schmelink Ramos Monterrosa, María Cristina Rozo Flórez, Angela Maritza Cajiao Pedraza, Ramón Ovidio García Rico , Gladys Montañez Acevedo
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
