Optimization of acetic acid Yield by gluconobacter oxydans using a Box-Behnken design
DOI:
https://doi.org/10.24054/limentech.v22i1.3184Keywords:
acetic acid bacteria, dairy wastewater, response surface methodologyAbstract
This study aims to optimize acetic acid (AA) production by Gluconobacter oxydans using a culture medium modified with dairy wastewater, applying a Box-Behnken design. The independent variables evaluated were glucose concentration, temperature (°C), and incubation time (hours). The resulting regression model demonstrated that temperature and incubation time were statistically significant factors influencing AA yield. Optimal conditions for maximum AA production were determined to be 33.484°C, 5.030% (v/w) glucose concentration, and 70.565 hours of incubation, yielding a predicted AA concentration of 4.763 g/100 mL. The relationships among these variables were modeled with a precision exceeding 91.58%, underscoring the robustness and reliability of the predictions. This optimized approach provides a sustainable method for AA production while repurposing dairy wastewater, contributing to a circular economy framework.
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