Genetic diversity in shiga toxin-producing escherichia colistrains isolated from ground meat of bovine origin
DOI:
https://doi.org/10.24054/limentech.v20i2.2272Keywords:
Meat, MLST, REP-PCR, Shiga ToxinAbstract
The main reservoir of the Shiga Toxin-Producing Escherichia colipathotype is cattle, making meat an important vehicle for its transmission to man. In order to characterize the Shiga Toxin-Producing Escherichia colistrains isolated from samples of this food, the phylogenetic classification of the strains was carried out, obtaining that they belonged to group E; Using the REP-PCR technique, a heterogeneous clonal pattern was detected; the MLST technique, on the other hand, identified new Type Sequences for each analyzed strain. No association was found between the characteristics of the meat samples and the strains analyzed.
Downloads
References
Álvarez-Suárez, M. E., Otero, A., García-López, M. L., Dahbi, G., Blanco, M., Mora, A., Blanco, J., & Santos, J. A. (2016). Genetic characterization of Shiga toxin-producing Escherichia coli (STEC) and atypical enteropathogenic Escherichia coli (EPEC) isolates from goat's milk and goat farm environment. International Journal of Food Microbiology, 236, 148–154. https://doi.org/10.1016/j.ijfoodmicro.2016.07.035
Bouzari, S., Jafari, A., & Aslani, M. (2012). Escherichia coli: A brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran. Iranian Journal of Microbiology, 4(3), 102–117.
Cadona, J. S., Bustamante, A. V., González, J., & Sanso, A. M. (2016). Genetic relatedness and novel sequence types of non-O157 Shiga toxin-producing Escherichia coli strains isolated in Argentina. Frontiers in Cellular and Infection Microbiology, 6, 93. https://doi.org/10.3389/fcimb.2016.00093
Cavalcanti, F., Hernandes, T., Takagi, E., Guth, C., Ori, É. de L., Pinheiro, S., De Andrade, S., Oliveira, S., Cergole, C., Francisco, G., & Dos Santos, L. (2020). Virulence profiling and molecular typing of Shiga toxin-producing E. coli (STEC) from human sources in Brazil. Microorganisms, 8(2). https://doi.org/10.3390/microorganisms8020171
Clermont, O., Christenson, J., Denamur, E., & Gordon, D. (2013). The Clermont Escherichia coli phylo-typing method revisited: Improvement of specificity and detection of new phylo-groups. Environmental Microbiology Reports, 5(1), 58–65. https://doi.org/10.1111/1758-2229.12019
Coura, F. M., de Araújo Diniz, S., Mussi, J., Silva, M. X., Lage, A. P., & Heinemann, M. B. (2017). Characterization of virulence factors and phylogenetic group determination of Escherichia coli isolated from diarrheic and non-diarrheic calves from Brazil. Folia Microbiologica, 62(2), 103–118. https://doi.org/10.1007/s12223-016-0480-9
De Campos, A., Puño-Sarmiento, J. J., Medeiros, L. P., Gazal, L., Maluta, R. P., Navarro, A., Kobayashi, R., Fagan, E. P., & Nakazato, G. (2018). Virulence genes and antimicrobial resistance in Escherichia coli from cheese made from unpasteurized milk in Brazil. Foodborne Pathogens and Disease, 15(2), 94–100. https://doi.org/10.1089/fpd.2017.2345
ECDC. (2019). The European Union One Health 2018 Zoonoses Report. European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA and ECDC). https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2019.5926
Furlan, J. P. R., da Silva Ferreira, M. E., & Stehling, E. G. (2020). Genetic diversity of multidrug-resistant CMY-producing Escherichia coli from feces and soil in a small-scale pig farm. Microbial Drug Resistance, 26(11), 1365-1371. https://doi.org/10.1089/mdr.2020.0090
Furlan, J., Gallo, I., de Campos, A., Navarro, A., Kobayashi, R., Nakazato, G., & Stehling, E. G. (2019). Characterization of non-O157 Shiga toxin-producing Escherichia coli (STEC) obtained from feces of sheep in Brazil. World Journal of Microbiology and Biotechnology, 35(9), 134. https://doi.org/10.1007/s11274-019-2712-z
García-Meniño, I., García, V., Alonso, M. P., Blanco, J. E., Blanco, J., & Mora, A. (2021). Clones of enterotoxigenic and Shiga toxin-producing Escherichia coli implicated in swine enteric colibacillosis in Spain and rates of antibiotic resistance. Veterinary Microbiology, 252, 108924. https://doi.org/10.1016/j.vetmic.2020.108924
Goudarztalejerdi, A., Mohammadzadeh, A., Najafi, S. V., Nargesi, F., & Joudari, S. (2020). Serogrouping, phylotyping, and virulence genotyping of commensal and avian pathogenic Escherichia coli isolated from broilers in Hamedan, Iran. Comparative Immunology, Microbiology and Infectious Diseases, 73, 101558. https://doi.org/10.1016/j.cimid.2020.101558
Gyles, C. (2007). Shiga toxin-producing Escherichia coli: An overview. Journal of Animal Science, 85, E45-E62.
Hasanpour, M., & Najafi, A. (2017). Development of a multiplex real-time PCR assay for phylogenetic analysis of uropathogenic Escherichia coli. Journal of Microbiological Methods, 137, 25–29. https://doi.org/10.1016/j.mimet.2017.03.009
Healy, M., Huong, J., Bittner, T., Lising, M., Frye, S., Raza, S., Schrock, R., Manry, J., Renwick, A., Nieto, R., Woods, C., Versalovic, J., & Lupski, J. R. (2005). Microbial DNA typing by automated repetitive-sequence-based PCR. Journal of Clinical Microbiology, 43(1), 199–207. https://doi.org/10.1128/JCM.43.1.199
Herrera, F., Santos, J., & Villamizar, R. (2019). Primer reporte de Escherichia coli productora de toxina Shiga no O157 que codifica el gen de la enterohemolisina en carne cruda en Colombia. Archivos Latinoamericanos de Nutrición, 69, 59–67.
Hoffmann, S. A., Pieretti, G. G., Fiorini, A., Patussi, E. V., Cardoso, R. F., & Mikcha, J. M. (2014). Shiga-toxin genes and genetic diversity of Escherichia coli isolated from pasteurized cow milk in Brazil. Journal of Food Science, 79(6), M1175–M1180. https://doi.org/10.1111/1750-3841.12477
ICMSF. (2005). Microorganisms in Foods 6: Microbial Ecology of Food Commodities (2nd ed.). New York: Kluwer Academic & Plenum Publishers.
Martínez, A. J., Bossio, C. P., Durango, A. C., & Vanegas, M. C. (2007). Characterization of Shiga toxigenic Escherichia coli isolated from foods. Journal of Food Protection, 70(12), 2843–2846. https://doi.org/10.4315/0362-028x-70.12.2843
Millán, Y., Hernández, E., Millán, B., & Araque, M. (2014). Distribución de grupos filogenéticos y factores de virulencia en cepas de Escherichia coli uropatógena productora de B-lactamasa CTX-M-15 aisladas de pacientes de la comunidad en Mérida, Venezuela. Revista Argentina de Microbiología, 46(3), 175–181. https://doi.org/10.1016/S0325-7541(14)70069-0
Ministerio de Salud y Protección Social de Colombia. (2015). Resolución 0719 de 11 de marzo. Por el cual se establece la clasificación de alimentos para consumo humano de acuerdo con el riesgo en salud pública.
Mohapatra, B., Broersma, K., & Mazumder, A. (2008). Differentiation of fecal Escherichia coli from poultry and free-living birds by (GTG)5-PCR genomic fingerprinting. International Journal of Medical Microbiology, 298(3–4), 245–252. https://doi.org/10.1016/j.ijmm.2007.03.019
Nazaret, E. (2009). Escherichia coli Shiga Toxigénica: Patogénesis, diagnóstico y tratamiento. Revista de La Sociedad Venezolana de Microbiología, 29(1), 13-20. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S1315-25562009000100004&lng=es&tlng=es
Pérez-Losada, M., Arenas, M., & Castro-Nallar, E. (2017). Multilocus sequence typing of pathogens. In M. Tibayrenc (Ed.), Genetics and Evolution of Infectious Diseases (pp. 383–404). Elsevier. https://doi.org/10.1016/B978-0-12-799942-5.00016-0
Rios, E., Santos, J., García, I., Flament, S., Blanco, J., García, M., Otero, A., & Rodríguez, J. (2019). Characterisation, antimicrobial resistance and diversity of atypical EPEC and STEC isolated from cow’s milk, cheese and dairy cattle farm environments. LWT, 108, 319–325. https://doi.org/10.1016/j.lwt.2019.03.062
Rojas-Lopez, M., Monterio, R., Pizza, M., Desvaux, M., & Rosini, R. (2018). Intestinal pathogenic Escherichia coli: Insights for vaccine development. Frontiers in Microbiology, 9, 440. https://doi.org/10.3389/fmicb.2018.00440
Rúgeles, L. C., Bai, J., Martínez, A. J., Vanegas, M. C., & Gómez-Duarte, O. G. (2010). Molecular characterization of diarrheagenic Escherichia coli strains from stool samples and food products in Colombia. International Journal of Food Microbiology, 138(3), 282–286. https://doi.org/10.1016/j.ijfoodmicro.2010.01.034
Silva, E., Leitão, S., Tenreiro, T., Pomba, C., Nunes, T., Lopes da Costa, L., & Mateus, L. (2009). Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows. Journal of Dairy Science, 92(12), 6000–6010. https://doi.org/10.3168/jds.2009-2358
Tack, D. M., Ray, L., Griffin, P. M., Cieslak, P. R., Dunn, J., Rissman, T., Jervis, R., Lathrop, S., Muse, A., Duwell, M., & Smith, K. (2020). Preliminary incidence and trends of infections with pathogens transmitted commonly through food - Foodborne Diseases Active Surveillance Network, 10 U.S. sites, 2016-2019. MMWR. Morbidity and Mortality Weekly Report, 69(17), 509–514. https://doi.org/10.15585/mmwr.mm6917a1
Urwin, R., & Maiden, M. C. (2003). Multi-locus sequence typing: a tool for global epidemiology. Trends in Microbiology, 11(10), 479–487. https://doi.org/10.1016/j.tim.2003.08.006
World Health Organization & Food and Agriculture Organization of the United Nations. (2018). Shiga Toxin-Producing Escherichia coli (STEC) and Food: Attribution, Characterization, and Monitoring: Report. https://www.who.int/publications/i/item/9789241514279
Wirth, T., Falush, D., Lan, R., Colles, F., Mensa, P., Wieler, L., Karch, H., Reeves, P., Maiden, M., Ochman, H., & Achtman, M. (2006). Sex and virulence in Escherichia coli: An evolutionary perspective. Molecular Microbiology, 60(5), 1136–1151. https://doi.org/10.1111/j.1365-2958.2006.05172.x
Downloads
Published
Versions
- 2022-01-12 (3)
- 2023-03-22 (2)
- 2023-02-01 (1)